Stem Cell and Xenograft Core

Overview of Services

The Stem Cell and Xenograft Core (SCXC) is a comprehensive resource that integrates:
a full range of murine in-vivo xenograft services + a viable tissue bank of normal human hematopoietic cells and hematopoietic malignancies.

The SCXC offers a wide variety of xenograft services from training to full-service experiments.
The Core maintains a large breeding colony of immune-deficient (NSG) mice for users' xenograft studies. We also offer humanized immune system NSG mice for a wide variety of studies ranging from gene therapy to HIV. Experimental animals are housed in a dedicated BSL-2 animal barrier space for severely immune deficient models. Currently established xenograft models include orthotopic tumor cell injections and renal capsule implantation, as well as normal human CD34 and leukemia engraftment, human iPS and ES-derived teratomas, and human skin grafting. We also offer access to a dedicated optical/fluorescence (IVIS Spectrum) imaging system located within the Core's BSL-2 space through our partnership with SAIF (Small Animal Imaging Facility).
For consultation or questions, please contact scxc@lists.upenn.edu 

The SCXC is committed to facilitating and promoting translational research involving viable primary human hematopoietic tissues.
Our core offers adult whole bone marrow from healthy donors and umbilical cord blood. Mononuclear and CD34⁺ cells from normal bone marrow and cord blood are available, and other cell fractions can be provided by arrangement. We maintain a large tissue bank of cells from hematopoietic malignancies including AML, ALL, CML, MDS and MPDs. All samples are fully annotated and frozen as viable cells. We also offer access to an immunomagnetic cell sorter (Miltneyi AutoMacs). Expertise in primary human hematopoietic stem/progenitor and leukemic cell culture and manipulation is available.

Research Resource identifier (RRID)

Our RRID is RRID:SCR_010035. Please cite us using this identifier when publishing your results.

Leadership

Tony Secreto, MS. - Associate Director, In-Vivo Services Coordinator

(215) 573-3705, fax:(215) 573-7049
asecreto@pennmedicine.upenn.edu

Martin Carroll, MD. - Medical Director
(215) 573-5712, fax:(215) 573-7049
carroll2@pennmedicine.upenn.edu 

Staff

Email for General Questions - scxc@lists.upenn.edu

Joshua Glover, LAT - Sr. Research Specialist
(215) 573-3705, fax:(215) 573-7049
aglover@pennmedicine.upenn.edu

Derrick Dopkin, LATG - Research Specialist
ddopkin@pennmedicine.upenn.edu

Eric Balboa, LATG - Resource Technologist
balboae@pennmedicine.upenn.edu 

Estefhany Davidson - Resource Technologist
estefhany.davidson@pennmedicine.upenn.edu

Gisela (Nikki) Brake-Sillá, M.F.A. - Project Manager
(215) 898-3974, fax:(215) 573-7049
gbrake@pennmedicine.upenn.edu

Michele Arlotta - Business Administrator
(215) 898-9615, fax:(215) 573-8282
michelea@pennmedicine.upenn.edu

Location and hours of operation

Links and Resources

1. http://www.med.upenn.edu/scxc/

Contacts