For system help please email Penn’s iLab support at: iLab.Support@pennmedicine.upenn.edu
(hide this warning on this page)The Electron Microscopy Resource Lab (EMRL) at the Perelman School of Medicine, University of Pennsylvania, is a training and service facility dedicated to providing both conventional transmission electron microscopy (TEM) of cells and tissues and state-of the art cryo electron microscopy (cryo-EM) and cryo electron tomography (cryo-ET) for structural investigation of macromolecules and cells. Our facility houses four transmission electron microscopes: JEM-1010 microscope for ultrastructure analysis; T12, TF20, and Krios G3i microscopes for cryo-EM/cryo-ET analysis. The core facility offers services to University of Pennsylvania research groups and external academic research groups in the greater Philadelphia area.
EMRL Faculty Director
Vera Moiseenkova-Bell, PhD
Associate Professor
Department of Systems Pharmacology and Translational Therapeutics
vmb@pennmedicine.upenn.edu
215-898-1154
Cryo-EM Screening and Training Facility:
is the part of the Electron Microscopy Resource Laboratory that provides training and access to state-of the art cryo electron microscopy (cryo-EM) and cryo electron tomography (cryo-ET) for structural investigation of macromolecules and cells. The facility houses two FEI microscopes (T12 and TF20), sample preparation instruments such as Vitrobot as well as two Leica cryo plungers, and all the accessories needed to perform sample screening using negative stain imaging and cryo-EM. The facility is available to Penn research groups and external academic research groups in the greater Philadelphia area.
The goal of The Cryo-EM Screening and Training Facility is to train users to self-sufficiency and proficiency in microscope operation and sample preparation with the ultimate goal of creating independent users who require minimal assistance from staff.
We offer the following services at the Cryo-EM Screening and Training Facility:
Note: The Cryo-EM Screening and Training Facility does not provide cryo-EM data processing and reconstruction services.
To request services please contact us at: SCREEN_SERV@lists.upenn.edu
Cryo-EM Screening and Training Facility Director
Sudheer Molugu, Ph.D.
Ultrastructure Lab:
The Ultrastructure Facility is the part of the Electron Microscopy Resource Laboratory that provides conventional transmission electron microscopy (TEM) of cells and tissues services to Penn research groups and external academic research groups in the greater Philadelphia area. The facility houses a JEOL JEM-1010 microscope, sample preparation instruments and all the accessories needed to perform fixing, processing, sectioning, staining, and imaging.
We offer the following services at the Ultrastructure Facility:
Note: The Ultrastructure Facility does not provide data analysis or processing.
To request services please contact us at: ULTRA_SERV@lists.upenn.edu
Ultrastructure Facility Technical Director
Biao Zuo, M.D., M.S.
Ultrastructure Facility Research Specialist
Inna Martynyuk, M.S.
Our RRID is: RRID:SCR_022375. Please cite us using this identifier when publishing your results.
Hours | Location |
9 am - 5 pm Monday - Friday |
3620 Hamilton Walk B39 Anatomy and Chemistry Philadelphia, PA 19104 |
Services List |
► Cryo-EM lab services (2) | |||
Name | Description | Price | |
---|---|---|---|
Sample Imaging (performed by staff) |
|
Internal
$150.00
/hr
Academic (External) $240.00 /hr Corporate (External) $300.00 /hr |
|
T12 Microscope training |
* Here, you will learn T12 TEM operation, Negative staining technique, safe operation of Microscope and holders. * You are required to purchase TEM grids for negative staining. Click Here and find CF300-Cu and add to cart. *This service fee is to reserve time of the expert trainer for supervision, training and teaching a user 2 sessions (4 hrs each). * Training is available on Monday and Tuesday 1 PM to 5 PM only. *This training is a pre-requisite and required for every user before requesting Cryo-EM training. |
Inquire | |
► Ultrastructure lab services (14) | |||
Name | Description | Price | |
Critical Point Dryer |
Internal
$20.00
each
Academic (External) $28.00 each Corporate (External) $40.00 each |
||
Duplicate block for post embedding IEM | Inquire | ||
Duplicate block for TEM analysis |
Internal
$175.00
each
Academic (External) $245.00 each Corporate (External) $350.00 each |
||
Each additional antibody | Inquire | ||
Jeol 1010 Imaging performed by staff | Inquire | ||
Jeol 1010 Training | Inquire | ||
Post-Embedded IEM | Inquire | ||
Reichart Ultramicrotome Training | Inquire | ||
Thick Sectioning only (existing block) | Inquire | ||
Duplicate Block for Post-Embedding IEM |
*pricing is per sample |
Inquire | |
Routine Analyses TEM |
*pricing is per sample |
Inquire | |
Specimen Processing Only |
*pricing is per sample |
Inquire | |
Thick Sectioning only |
(existing block) *pricing is per sample
|
Inquire | |
Thin Sectioning Only | Inquire |