Electron Microscopy Resource Laboratory:

The Electron Microscopy Resource Lab (EMRL) at the Perelman School of Medicine, University of Pennsylvania, is a training and service facility dedicated to providing both conventional transmission electron microscopy (TEM) of cells and tissues and state-of the art cryo electron microscopy (cryo-EM) and cryo electron tomography (cryo-ET) for structural investigation of macromolecules and cells. Our facility houses four transmission electron microscopes: JEM-1010 microscope for ultrastructure analysis; T12, TF20, and Krios G3i microscopes for cryo-EM/cryo-ET analysis. The core facility offers services to University of Pennsylvania research groups and external academic research groups in the greater Philadelphia area.

EMRL Faculty Director
Vera Moiseenkova-Bell, PhD
Associate Professor
Department of Systems Pharmacology and Translational Therapeutics
vmb@pennmedicine.upenn.edu
215-898-1154

Cryo-EM Screening and Training Facility: 

is the part of the Electron Microscopy Resource Laboratory that provides training and access to state-of the art cryo electron microscopy (cryo-EM) and cryo electron tomography (cryo-ET) for structural investigation of macromolecules and cells. The facility houses two FEI microscopes (T12 and TF20), sample preparation instruments such as Vitrobot as well as two Leica cryo plungers, and all the accessories needed to perform sample screening using negative stain imaging and cryo-EM. The facility is available to Penn research groups and external academic research groups in the greater Philadelphia area.

The goal of The Cryo-EM Screening and Training Facility is to train users to self-sufficiency and proficiency in microscope operation and sample preparation with the ultimate goal of creating independent users who require minimal assistance from staff.

We offer the following services at the Cryo-EM Screening and Training Facility:

• Training on the two FEI microscopes (T12 and TF20) for negative stain imaging and cryo-EM.
• Training on sample preparation instruments such as Vitrobot and two Leica cryo plungers.
• Pay-per-service for negative stain imaging. Note that this service is only available for Penn and CHOP users.

Note: The Cryo-EM Screening and Training Facility does not provide cryo-EM data processing and reconstruction services.

To request services please contact us at: SCREEN_SERV@lists.upenn.edu

Cryo-EM Screening and Training Facility Director
Sudheer Molugu, Ph.D.

Ultrastructure Lab:

The Ultrastructure Facility is the part of the Electron Microscopy Resource Laboratory that provides conventional transmission electron microscopy (TEM) of cells and tissues services to Penn research groups and external academic research groups in the greater Philadelphia area. The facility houses a JEOL JEM-1010 microscope, sample preparation instruments and all the accessories needed to perform fixing, processing, sectioning, staining, and imaging.

We offer the following services at the Ultrastructure Facility:

• Pay-per-service Standard TEM and Immuno TEM for cellular and tissue structure. 
• Pay-per-service specimen preparation included fixing, processing, sectioning, staining, and imaging.
• Training on the Reichart Ultramicrotome for independent usage.
• Training on the JEOL 1010 microscope for independent microscope usage.

Note: The Ultrastructure Facility does not provide data analysis or processing.

To request services please contact us at: ULTRA_SERV@lists.upenn.edu

Ultrastructure Facility Technical Director
Biao Zuo, M.D., M.S.

Ultrastructure Facility Research Specialist
Inna Martynyuk, M.S.

Research Resource Identifier (RRID)

Our RRID is: RRID:SCR_022375. Please cite us using this identifier when publishing your results. 

Location and hours of operation

 Links and Resources

1. UPenn Electron Microscopy Resource Laboratory