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CRISPR/Cas9 Mouse Targeting Core (UPenn)

 

*MISSION STATEMENT: The CRISPR/Cas9 mouse targeting core at the Perelman School of Medicine, University of Pennsylvania, has been operational since February of 2017. Prof. Jorge HENAO-MEJIA, M.D., Ph.D. has served as Scientific Director and Leonel JOANNAS, LATg, as the Technical Director. The core is located in the Institute for Immunology (IFI) and the Scientific and Technical Directors are part of the IFI. In addition, this recently established core is composed of one research technologist “A” who works under our guidance. The mission of the CRISPR/Cas9 targeting core is to streamline procedures to facilitate the use of the CRISPR/Cas9 genome editing technology by the larger UPenn/CHOP community to rapidly and economically generate novel mouse genetic tools. This core has had a significant positive impact upon the community. Researchers who work with mice and rats to model human diseases have enthusiastically taken up these tools. Projects that may have taken years using older techniques, such as examining the effects of disrupting several genes at once, generating point mutations or inserting fluorescent proteins, now take just a couple months. It is also easier to produce more subtle changes, like tweaking just a few DNA nucleotides in a gene in a way that simulates a variation found in humans. In addition, our R&D efforts in this area should enable UPenn/CHOP to remain at the forefront of this technology.

 

*SUMMARY OF ACTIVITIES: By using the CRISPR/Cas9 technology, we can now generate pre-clinical mouse and rat models for research purposes in a matter of 3 to 4 weeks. Moreover, it allows us to generate models that more closely mimic human diseases. Hence, this truly has been a revolution in the biomedical research field and its impact is already observed in the clinics. We have already developed more than 200 different targeting strategies during our first 2 and half years of being operational. The CRISPR/Cas9 Mouse Targeting Core at Penn is a state-of-the-art facility that has an outstanding record of accomplishment in the production of genetically altered mice and rats for investigators at Penn and outside institutions, within and outside the state of Pennsylvania, as well as overseas. 

 

*IMPORTANT: ALL SERVICES AND MICE ARE PROVIDED “AS IS,” WITHOUT ANY EXPRESS OR IMPLIED WARRANTIES, AND THE CORE AND UNIVERSITY OF PENNSYLVANIA HEREBY EXPRESSLY EXCLUDE ANY WARRANTY OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR TITLE. YOU HEREBY AGREE THAT THE UNIVERSITY OF PENNSYLVANIA AND THE CORE’S SOLE LIABILITY, AND YOUR SOLE AND EXCLUSIVE REMEDY, FOR ANY ALLEGED ACT, OMISSION, NEGLIGENCE OR OTHER WRONG IN PERFORMING SERVICES, IS THE REPEAT OF THE PARTICULAR SERVICE. YOU HEREBY AGREE THAT THE UNIVERSITY AND ITS CORE AND ITS PERSONNEL ARE NOT LIABLE TO YOU OR ANYONE ELSE FOR ANY MONETARY DAMAGES, WHETHER DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR OTHER TYPE OF MONETARY DAMAGES. BY PLACING AN ORDER FOR SERVICES, YOU AGREE TO PAY THE FEES CHARGED AND YOU AGREE TO ALL OF THE TERMS HERE DESCRIBED. Our main goal has always been to streamline procedures that facilitate investigators' use of the CRISPR/Cas9 genome editing technology for the rapid and economic generation of novel mouse genetic tools. However, we want to stress one more time that the CRISPR/Cas9 Mouse Targeting Core at the University of Pennsylvania provides an academic service to the community, and even though our overall rate of success is high, NEITHER A SUCCESSFUL TARGETING NOR GERM-LINE TRANSMISSION CAN BE GUARANTEED. We strongly advise our users to seek the services of a company when a POSITIVE TARGETING MUST BE GUARANTEED in advance.

 

*ONLY e-mail INQUIRIES, PLEASE! Do not submit your request for CRISPR/Cas9 services before meeting with us!

 

*OVERVIEW OF SERVICES - OFFERED CRISPR/Cas9 TARGETING STRATEGIES (LAST UPDATE ON 11/15/2019)

 

 

*CORE MEMBERS

Jorge HENAO-MEJIA, M.D., Ph.D.; Scientific Director

Leonel D. JOANNAS, LATg; Technical Director

Matthew MING; BSc. Research Technologist & Screening Specialist

 

*LOCATION

421 Curie Boulevard

Biomedical Research Building II/III, 3rd Floor, Room # 312 (Office) and Room # 320 (Laboratory)

Philadelphia, PA 19104, USA

Contacts

Name Role Phone Email Location
Jorge HENAO-MEJIA, M.D., Ph.D.
Scientific Director CRISPR/Cas9 Mouse Targeting Facility
 
Only e-mail inquiries!
 
jhena@pennmedicine.upenn.edu
 
BRB II/III, 3rd Floor, Room # 312
 
Leonel D. JOANNAS, LATg
Technical Director CRISPR/Cas9 Mouse Targeting Facility
 
Only e-mail inquiries!
 
ljoannas@pennmedicine.upenn.edu
 
BRB II/III, 3rd Floor, Room # 320
 
Matthew MING, BSc.
Research Technologist & Screening Specialist
 
Only e-mail inquiries!
 
matthew.ming@pennmedicine.upenn.edu
 
BRB II/III, 3rd Floor, Room # 320
 

Map


https://www.google.com/maps/place/Biomedical+Research+Building+II%2FIII,+421+Curie+Blvd,+Philadelphia,+PA+19104/@39.9478702,-75.1990227,17z/data=!3m1!4b1!4m5!3m4!1s0x89c6c65ec9c3306f:0x810d59d739a86378!8m2!3d39.9478702!4d-75.196834