CRISPR-Cas9 Mouse Targeting Core (UPenn)

MISSION STATEMENT (2017-2024)

The CRISPR-Cas9 mouse targeting core at the Perelman School of Medicine, University of Pennsylvania, has been operational since February 2017. Jorge HENAO-MEJIA has served as the Scientific Director and Leonel JOANNAS as the Technical Director. The core is in the Institute for Immunology (IFI) and the Scientific and Technical Directors are part of the IFI. In addition, this recently established core is composed of one research technologist “A” who works under our guidance. The mission of the CRISPR-Cas9 targeting core is to streamline procedures to facilitate the use of the CRISPR/Cas9 genome editing technology by the larger UPenn/CHOP community to generate novel mouse genetic tools rapidly and economically. This core has had a significant positive impact on the community. Researchers who work with mice and rats to model human diseases have enthusiastically taken up these tools. Projects that may have taken years using older techniques, such as examining the effects of disrupting several genes at once, generating point mutations, or inserting fluorescent proteins, now take just a couple of months. It is also easier to produce more subtle changes, like tweaking just a few DNA nucleotides in a gene in a way that simulates a variation found in humans. In addition, our R&D efforts in this area should enable UPenn/CHOP to remain at the forefront of this technology.

RESEARCH RESOURCE IDENTIFIER (RRID)

Our RRID is: RRID:SCR_022378. Please cite us using this identifier when publishing your results!

SUMMARY OF ACTIVITIES (2017-2024)

By using the CRISPR/Cas9 technology, we can now generate pre-clinical mouse and rat models for research purposes in a matter of 3 to 4 weeks. Moreover, it allows us to generate models that more closely mimic human diseases. Hence, this truly has been a revolution in the biomedical research field, and its impact is already observed in the clinics. We have already developed more than two hundred different targeting strategies during our first two and a half years of being operational. The CRISPR-Cas9 Mouse Targeting Core at Penn is a state-of-the-art facility that has an outstanding record of accomplishment in the production of genetically altered mice and rats for investigators at Penn and outside institutions, within and the state of Pennsylvania, as well as overseas.

IMPORTANT (MANDATORY TERMS, 2017-2024)

1. ALL SERVICES AND MICE ARE PROVIDED “AS IS,” WITHOUT ANY EXPRESS OR IMPLIED WARRANTIES, AND THE CORE AND THE UNIVERSITY OF PENNSYLVANIA HEREBY EXPRESSLY EXCLUDE ANY WARRANTY OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NON-INFRINGEMENT, OR TITLE.

2. YOU HEREBY AGREE THAT THE UNIVERSITY OF PENNSYLVANIA AND THE CORE’S SOLE LIABILITY, AND YOUR SOLE AND EXCLUSIVE REMEDY, FOR ANY ALLEGED ACT, OMISSION, NEGLIGENCE, OR OTHER WRONG IN PERFORMING SERVICES, IS THE REPEAT OF THE PARTICULAR SERVICE.

3. YOU HEREBY AGREE THAT THE UNIVERSITY AND ITS CORE AND ITS PERSONNEL ARE NOT LIABLE TO YOU OR ANYONE ELSE FOR ANY MONETARY DAMAGES, WHETHER DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR OTHER TYPES OF MONETARY DAMAGES.

4. BY PLACING AN ORDER FOR SERVICES, YOU AGREE TO PAY THE FEES CHARGED AND YOU AGREE TO ALL OF THE TERMS DESCRIBED HERE.

5. OUR MAIN GOAL HAS ALWAYS BEEN TO STREAMLINE PROCEDURES THAT FACILITATE INVESTIGATORS' USE OF THE CRISPR/CAS9 GENOME EDITING TECHNOLOGY FOR THE RAPID AND ECONOMIC GENERATION OF NOVEL MOUSE GENETIC TOOLS. HOWEVER, WE WANT TO STRESS ONE MORE TIME THAT THE CRISPR/CAS9 MOUSE TARGETING CORE AT THE UNIVERSITY OF PENNSYLVANIA PROVIDES AN ACADEMIC SERVICE TO THE COMMUNITY, AND EVEN THOUGH OUR OVERALL RATE OF SUCCESS IS HIGH, NEITHER A SUCCESSFUL TARGETING NOR GERM-LINE TRANSMISSION CAN BE GUARANTEED. WE STRONGLY ADVISE OUR USERS TO SEEK THE SERVICES OF A COMPANY WHEN POSITIVE TARGETING MUST BE GUARANTEED IN ADVANCE.

Contacts

Map

https://www.google.com/maps/place/Biomedical+Research+Building+II%2FIII,+421+Curie+Blvd,+Philadelphia,+PA+19104/@39.9478702,-75.1990227,17z/data=!3m1!4b1!4m5!3m4!1s0x89c6c65ec9c3306f:0x810d59d739a86378!8m2!3d39.9478702!4d-75.196834