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(hide this warning on this page)The Stem Cell and Xenograft Core (SCXC) is a comprehensive resource laboratory that integrates a viable tissue bank of normal human hematopoietic cells and hematopoietic malignancies with a full range of xenograft services.
The SCXC is committed to facilitating and promoting translational research involving viable primary human hematopoietic tissues. Our core offers adult whole bone marrow from healthy donors and umbilical cord blood. Mononuclear and CD34+ cells from normal bone marrow and cord blood are available, and other cell fractions can be provided by arrangement. We maintain a large tissue bank of cells from hematopoietic malignancies including AML, ALL, CML, MDS and MPDs. All samples are fully annotated and frozen as viable cells. We also offer access to an immunomagnetic cell sorter (Miltneyi AutoMacs). Expertise in primary human hematopoietic stem/progenitor and leukemic cell culture and manipulation is available. For consultation or questions, please contact Martin Carroll.
The SCXC offers a wide variety of xenograft services from training to full-service experiments. The Core maintains a large breeding colony of immune-deficient (NSG) mice for users xenograft studies. We also offer human immune system (CD34 - transplanted) NSG mice for a wide variety of studies ranging from gene therapy to HIV. Experimental animals are housed in dedicated BSL-2 animal barrier space equipped for whole body irradiation and all necessary procedures and survival surgeries. Currently established xenograft models include normal human CD34 and leukemia engraftment, human iPS and ES-derived teratomas, human skin grafting, orthotopic human ovarian, hepatic and pancreatic tumor cell injections, renal capsule implantation. We also offer access to a dedicated optical/fluorescence (IVIS Spectrum) imaging system located within the Core's BSL-2 space. For consultation or questions, please contact Nicolas Skuli, Ph.D.
Our RRID is RRID:SCR_010035. Please cite us using this identifier when publishing your results.
Nicola Skuli, Ph.D. - Director
(215) 746-0181, fax:(215) 573-7049
nicskuli@pennmedicine.upenn.edu
Martin Carroll, MD. - Medical Director
(215) 573-5712, fax:(215) 573-7049
carroll2@pennmedicine.upenn.edu
Tony Secreto- In-Vivo Services Coordinator
(215) 573-3705, fax:(215) 573-7049
asecreto@pennmedicine.upenn.edu
Michele Arlotta- Business Administrator
(215) 898-9615, fax:(215) 573-8282
michelea@pennmedicine.upenn.edu
Joshua Glover- Research Specialist
(215) 573-3705, fax:(215) 573-7049
aglover@pennmedicine.upenn.edu
Gisela (Nikki) Brake-Sillá, M.F.A.- Project Manager
(215) 898-3974, fax:(215) 573-7049
gbrake@pennmedicine.upenn.edu
Derrick Dopkin- Research Specialist
ddopkin@pennmedicine.upenn.edu
Eric Balboa - Resource Technologist
balboae@pennmedicine.upenn.edu
| Hours | Location |
|
Facility Hours: 24/7 Staff Hours: Variable |
BRB II/III, 7th Floor 421 Curie Blvd. University of Pennsylvania Philadelphia, PA 19104-6160 Telephone:(215) 746-0181 Fax:(215) 573-8282
Click here to access map |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Nicolas Skuli |
Director
|
(215) 746-0181 fax:(215) 573-7049
|
nicskuli@pennmedicine.upenn.edu
|
710 BRB
|
| Michele Arlotta |
Business Administrator
|
215-898-9615
|
michelea@pennmedicine.upenn.edu
|
811 BRB
|
| Service list |
| ► Tissue Bank - Healthy Donor (5) | |||
| Name | Description | Price | |
|---|---|---|---|
| Cord Blood CD34 Negs (per million) |
Internal
$5.00
each
Academic (External) $7.50 each Corporate (External) $10.00 each |
||
| Cord Blood Mononuclear Cells (per million) |
Internal
$10.00
each
Academic (External) $12.50 each Corporate (External) $20.00 each |
||
| Fresh CD34+ from Cord Blood ( per million) |
Internal
$550.00
each
Academic (External) $825.00 each Corporate (External) $1,100.00 each |
||
| Fresh Cord Blood (per ml) |
Internal
$3.00
each
Academic (External) $4.50 each Corporate (External) $6.00 each |
||
| Frozen CD34+ from Cord Blood ( per million) |
Internal
$635.00
each
Academic (External) $953.00 each Corporate (External) $1,270.00 each |
||
| ► Tissue Bank - Hematologic Malignancy Patient (2) | |||
| Name | Description | Price | |
| AML - Colony Forming Cell (CFC) Assay |
Internal
$110.00
plate
Academic (External) $176.00 plate Corporate (External) $220.00 plate |
||
| Patient Specimen Fresh (PB or BM) per tube | Inquire | ||
| ► Xenograft (3) | |||
| Name | Description | Price | |
| Animal Receiving |
Per crate (max 20 animals) |
Internal
$55.00
each
Academic (External) $82.50 each Corporate (External) $110.00 each |
|
| Receptor Enumeration (per sample) |
Internal
$43.00
each
Academic (External) $65.00 each Corporate (External) $86.00 each |
||
| Tissue Harvest, STERILE (bone marrow, spleen, cardiac blood) per animal |
Internal
$78.00
each
Academic (External) $117.00 each Corporate (External) $156.00 each |
||
