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About

Beckman Center for Cryo-Electron Microscopy

 

Please contact us if you have a sample for screening or data collection on the Titan Krios and/or Glacios 2 by sending an email at cryo-EM@lists.upenn.edu. Visit our website for more information Beckman Center for Cryo-EM

 

Overview of Services

The Beckman Center for Cryo-Electron Microscopy provides access to advanced cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) technologies for high-resolution structural analysis of macromolecules both in vitro and in situ. The facility is equipped with a 300 keV Titan Krios G3i featuring a phase plate, K3 Summit direct electron detector, and BioQuantum GIF energy filter. It also houses a 200 keV Glacios 2 equipped with a Falcon 4i detector and Selectris energy filter.
 
For sample preparation, the center offers a Vitrobot cryo-plunger along with all required accessories to support high-quality grid preparation. The facility is open to research groups at the University of Pennsylvania, external academic institutions and corporate users in the greater Philadelphia area.
 
The cryo-ET branch provides specialized expertise and instrumentation for advanced sample preparation workflows as well. This includes a Zeiss LSM 900 confocal microscope to support correlative cryogenic workflows and targeted cryo-ET data acquisition. Additionally, a Zeiss Crossbeam 550 (equipped with a cryostage, a Delmic Meteor in-chamber fluorescence microscope, high signal-to-noise primary and secondary electron detectors and superior electron optics) is capable of conducting volumetric scanning electron microscopy (vSEM) reconstructions and thinning of biological specimens in a targeted fashion for subsequent cryo-ET. 
 
For cryo-EM related requests and/or inquiries, contact cryo-em@lists.upenn.edu
For cryo-ET–related requests and/or inquiries, contact cryo-et@lists.upenn.edu
 
Advanced cryo-EM:
 
  • Freezing, screening and optimization of samples for cryo-EM (single particle analysis and LNPs).
  • High resolution SPA data acquisition of the prescreened samples on the Glacios 2 or Krios G3i microscope (based on availability).
  • Hands-on training is provided for all stages of the workflow, from sample preparation to data collection.
  • Hands-off MicroED service for small-molecule crystals and protein microcrystals.
  • Data transfer via the Penn network (internal users only), hard drives or file sharing services such as Globus (user needs to provide account).
Note: The “advanced cryo-EM” branch does not provide cryo-EM data processing and reconstruction services. However, we may be able to assist - please inquire if you need help with data processing. 
 
Advanced cryo-ET:
 
  • Training and data acquisition on the LSM900.
  • Training and data acquisition on the Crossbeam 550.
  • Assistance with sample preparation for cryo-ET
  • Assistance with and training on the Krios for cryo-ET data acquisition
  • Assistance with cryo-ET data processing and post processing/analyses on a case-by-case basis.

 

Research Resource Identifier (RRID)

Our RRID is RRID:SCR_022375. Please cite us using this identifier when publishing your results. 

 

Faculty Director

Vera Moiseenkova-Bell, Ph.D.

Professor of Pharmacology

Department of Systems Pharmacology and Translational Therapeutics

 

Core Directors/Managers

Advanced cryo-EM: 

Prerana Gogoi, Ph.D.

prerana.gogoi@pennmedicine.upenn.edu

 

Julia Seifermann, Ph.D.

Julia.Seifermann@PennMedicine.upenn.edu

 

Advanced cryo-ET:

Shrawan Kumar Mageswaran, Ph.D.

shrawan.mageswaran@pennmedicine.upenn.edu

 

Elaine Mihelc, Ph.D.

elaine.mihelc@pennmedicine.upenn.edu

 

Hours 

24/7

 

Location

Krios and Glacios:

3205 Walnut St.

G011 Singh Center

Philadelphia, PA 19104 

 

LSM900 and Crossbeam 550:

B33A Anatomy Chemistry Building

3620 Hamilton Walk

Philadelphia, PA 19104

  

Links and Resources
For negative staining, ultrastructural traditional TEM, approach Electron Microscopy Resource Lab Website

Contacts

Name Role Phone Email Location
Vera Moiseenkova-Bell
Faculty Director
 
215-898-1154 
 
vmb@pennmedicine.upenn.edu
 

 
Ingrid Schafroth
Core Facilities Coordinator
 
215-898-8022
 
Ingrid.Schafroth@pennmedicine.upenn.edu
 
242 Anat-Chem Bldg.
 
Prerana Gogoi
Core Director
 

 
prerana.gogoi@pennmedicine.upenn.edu
 

 
Shrawan Kumar Mageswaran
Cryo-ET Technical Director
 

 
shrawan.mageswaran@pennmedicine.upenn.edu
 

 
Elaine Mihelc
Cryo-ET Technical Director
 

 
elaine.mihelc@pennmedicine.upenn.edu
 

 
Julia Seifermann
Postdoctoral Researcher
 

 
Julia.Seifermann@PennMedicine.upenn.edu
 

 

Service list


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