The Beckman Center for Cryo-Electron Microscopy is the part of the Electron Microscopy Resource Laboratory that provides access to state-of the art cryo electron microscopy (cryo-EM) and cryo electron tomography (cryo-ET) for structural investigation of macromolecules and cells. The facility houses a Krios G3i microscope equipped with phase plate, K3 Summit Direct Detector camera and Bioquantum GIF energy filter. In addition, the facility has Vitrobot cryo plunger and all accessories needed to perform sample preparation for cryo-EM. The facility is available to Penn research groups and external academic research groups in the greater Philadelphia area.

The Beckman Center for Cryo-Electron Microscopy is reserved for samples that have already been optimized and are ready for extended cryo-EM data collection sessions on the Krios G3i microscope. If your samples are still in the optimization stage, we refer you to our Cryo-EM Screening and Training Facility.

We offer the following services at the Beckman Center for Cryo-Electron Microscopy:

  • High resolution data acquisition of the prescreened Krios-qualified samples on the Krios G3i microscope.
  • Data transfer to the data cluster for internal users or hard drives for external users.
  • The facility is currently restricted to staff for operation and management of the microscope.

Note: The Beckman Center for Cryo-Electron Microscopy does not provide cryo-EM data processing and reconstruction services.

To request services please contact us at: BECKMAN_SERV@lists.upenn.edu

 

Beckman Center for Cryo-Electron Microscopy Faculty Director
Vera Moiseenkova-Bell, Ph.D.
Associate Professor
Department of Systems Pharmacology and Translational Therapeutics

Beckman Center for Cryo-Electron Microscopy Technical Director
Darrah Johnson-McDaniel, Ph.D.