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Proteomics Core

Overview of Services


The Children’s Hospital of Philadelphia Research Institute (CHOP) Proteomics Core Facility (PCF) provides state-of-the-art proteomics services for investigators at CHOP, University of Pennsylvania, and outside institutions.     A wide range of state-of-the-art quantitative proteomics experiments comprise the core of our services. These include but are not limited to post-translational modification analysis, protein identification, and targeted (e.g. co-immunoprecipitation) or comprehensive (e.g. protein expression profiling) proteome analysis.  Proteome analysis may employ a number of different isotope-labeling strategies to enable quantitative measurements for deep proteome coverage or multiplexed targeted analysis of protein abundances.  Quantitative analysis of phosphoproteomes, lysine acetylomes, ubiquitylomes and glycoproteomes together with whole proteome analysis are some of our signature areas of expertise.   An efficient and comprehensive urine proteomic analysis pipeline is also well established in the lab.  At the heart of all we do, are detailed quality control procedures to assure consistent peak performance of sample preparation and instrument analytical performance.  Workflows for all the aforementioned proteome analyses are unique to each project and can involve optimization of a range of multi-dimensional separation techniques coupled to the appropriate mass spectrometer.  For all but the most routine services, a strong interaction with PCF staff is encouraged throughout all phases of a project: planning, execution, and data reduction/interpretation.  A full range of statistical and bioinformatics analysis can be tailored to the specific needs of larger, more involved projects as required.


Note: Please use the 'Request Services' tab to contact us. ('Please contact me' checkbox then scroll down to bottom of page and 'Submit request to Core').




Lynn Spruce | Interim Technical Director

Location and hours of operation

Hours Location

M-F 10-5 

806 ARC

3615 Civic Center Blvd

Philadelphia, PA 19104

Links and Resources

  1. Proteomics Core Website


Name Role Phone Email Location
Lynn Spruce
Research Lab Manager
806 ARC
Hua Ding
Research Associate II
806 ARC
Hossein Fazelinia
Bioinformatics Scientist III
806 ARC
Jennifer Roof
Research Technician III
806 ARC
Elizabeth Brown
Research Technician II
806 ARC
Erik Nash
Research Technician II
806 ARC