For system help please email Penn’s iLab support at: iLab.Support@pennmedicine.upenn.edu
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The Penn Genomics and Sequencing Core (PGSC) provides a spectrum of sequencing, genomics and molecular biological services with a team of highly experienced and trained professionals under a Director and Technical Director. The services include consultation on experimental design to performing experiment to downstream data analyses.
We offer Next-Generation Sequencing on multiple platforms:
Short Read - Illumina NovaSeq X Plus, NextSeq 2000 and MiSeq
Long Read - PacBio Revio and Oxford Nanopore MinION
This includes DNA/RNA extraction from different sources, library preparation with quality control, and sequencing followed by bioinformatics.
Applications include mRNA-Seq, total RNA-Seq, small RNA-Seq, WGS, targeted sequencing, ChIP-Seq on X Plus; WGS and whole methylome on PacBio etc.
For single-cell genomics we use 10X Genomics Controller; the applications include scRNA-Seq and snRNA-Seq. Soon we are going to introduce multiplexed scRNA-Seq. Multiome including scATAC-Seq are under development.
Bioinformatics support is available for both core and externally generated datasets.
Legacy Sequencing: Sanger Sequencing on ABI 3730xl capillary sequencer.
The capillary sequencer enables fragment analysis for microsatellite-based genotyping, human cell line authentication and other applications.
Targeted molecular profiling including genotyping and gene expression is performed using ThermoFisher QS6 Pro and Qiagen digital PCR instruments. Mouse genotyping is done using PCR-based approach.
For further information please contact Director, Tapan Ganguly, gangulyt@pennmedicine.upenn.edu and Technical Director, Erik Toorens, toorens@pennmedicine.upenn.edu.
Charges for Major NGS Services
Informatics Services
The NGSC offers informatics services:
Please contact John Tobias – He will provide the service regardless of where the data is generated.
These other fine folks also provide service:
DOM DRC Functional Genomics Core - Jonathan Schug and Kirill Batmanov - Members of the Institute for Diabetes and Metabolism's (IDOM) Diabetes Research Center (DRC) can get a wide range of bioinformatics services via the Functional Genomics Core (FGC).
IBI - Bioinformatics Core - Taehyong Kim - The Bioinformatics Core in the Institute for Biomedical Informatics offers a wide range of services. See the BiC website for more information.
Our RRID is RRID:SCR_024999. Please cite us using this identifier when publishing your results.
Try these new links for help before, during, and after working with the NGSC.
PSOM iLab help - links to help for BAs and PIs
FAQs - answers to common questions.
URLs - links into the websites of Illumina, 10X Genomics, and others
We use iLab for equipment scheduling, starting an experiment, and for billing.
The science, quality checks, data delivery, FAQs, etc. is at our NGSC Website.
Accounts for iLab and the NGSC data website are distinct and must be set up separately for PI and investigator.
Contact us for letters of support for grant applications.
NGSC Websites
We are using iLab for equipment scheduling, starting an experiment, and for billing.
The science, quality checks, data delivery, etc. remains at our NGSC Website.
You will still need to use your NGSC accounts.
Take a look at our FAQ to see how we work.
Drop-off Hours | Location |
11:00AM-12:00PM - Tuesday to Thursday
2:30PM - 3:30PM - Monday to Friday
|
12-160 Smilow |
Make your remittance payable to Trustees of the University of Pennsylvania, Attn: Jeffrey Griffin.Other details pending.
Name | Role | Phone | Location | |
---|---|---|---|---|
Tapan Ganguly, Ph.D. |
Director
|
215-573-7238
|
gangulyt@pennmedicine.upenn.edu
|
B16 Anat-Chem Bldg.
|
Erik Toorens |
Technical Director
|
251-573-4832
|
toorens@pennmedicine.upenn.edu
|
502 Stellar-Chance Labs
|