Penn Genomic Analysis Core - DNA Sequencing Laboratory

 

Penn Genomic Analysis Core will be opening for all services on June 8st, 2020 in accordance with the guidance from Perelman School of Medicine’s Phase I reopening. During this time we will be accepting samples for Sanger, molecular biology work, NGS, BioAnalyzer, fragment analysis, cell culture work, microarray and real-time PCR. If you plan on submitting samples please work with us by scheduling a time to drop off samples through email for all RNA BioAnalyzer, DNA preps and any samples that cannot be delivered to the drop boxes. Sanger samples, Fragment Analysis, and DNA BioAnalyzer samples should be delivered to our drop boxes in BRB lobby, Johnson Pavilion lobby, and Smilow lobby in a small plastic bag with printout of submission. Samples will be picked up at 11AM every weekday. We kindly ask that you do not enter our lab. Please knock on the glass outside the lab so we can meet you in the hallway to pass samples to us. Please email the following people for sample drop off instructions and times:

Please use the link below to access our Sanger Submission site:

UPENN SANGER SUBMISSION SITE

 

Fragment Analysis and Genotyping, Human Cell Line Authentication: Jennifer Rosado

jmrosado@pennmedicine.upenn.edu

 

Mouse Genotyping, MaxiPrep, MiniPrep, and Molecular Biology work: Peiqin Chen

pechen@pennmedicine.upenn.edu

 

NGS, BioAnalyzer, Pippin, Qubit: Erik Toorens and Jennifer Rosado 

toorens@pennmedicine.upenn.edu

jmrosado@pennmedcine.upenn.edu

 

Microarray and Real-Time PCR: Hetty Rodriquez 

hettymr@pennmedicine.upenn.edu

 

All other inquiries or questions about our services please contact:

Tapan Ganguly PhD (Facility Director) gangulyt@pennmedicine.upenn.edu

Erik Toorens (Lab Manager) toorens@pennmedicine.upenn.edu

 

 

Overview of Services

DNA Sequencing Facility was founded in 1994 as an Abramson Cancer Center (ACC) core. In 2014, the Perelman School of Medicine (PSOM) and Abramson Cancer Center (ACC) combined two NCI CCSG supported cores, the DNA Sequencing Facility and the Molecular Profiling Facility to consolidate genomic services under a single umbrella Core. The resulting Shared Resource, the Penn Genomic Analysis Core was and continues to be directed by Dr. Tapan Ganguly.


DNA Sequencing Facility offers sequencing services on three platforms, gold standard Sanger sequencing on ABI capillary sequencers, next-generation sequencing (NGS) on Ion Torrent PGM and S5 and, Illumina MiSeq and Next-Seq. along with experimental design and data analysis.


The NGS service includes library preparation for multiple applications including targeted sequencing, RNA-Seq and Exome-Seq. Free consultation is provided for project planning and experimental design. Data analysis service is performed in consultation with the investigators.


The capillary sequencers also enable microsatellite genotyping and fragment analysis for VNTR, SNaPshot and Human Cell Line Authentication.


The molecular biological services include PCR, cloning, subcloning, mutagenesis, construct preparation and, plasmid DNA preps at different scales.

 

Guide to Ordering Services

Details of how to use iLab to order services are available in this Guide for Penn investigators. External users should use the guide for CHOP, Wistar, Monell, off-campus, for-profits. 

 

Leadership and Staff

Name Role Phone Email Location
Tapan Ganguly, Ph.D Director 215-573-7238 gangulyt@pennmedicine.upenn.edu

B16 Anat-Chem Bldg.

 

Erik Toorens Lab Manager 215-573-4832 toorens@pennmedicine.upenn.edu 502-503 Stellar-Chance Bldg.
Peiqin Chen Research Specialist 215-573-7407 pechen@pennmedicine.upenn.edu 502-503 Stellar-Chance Bldg.
Lan Ping Zhang Research Specialist 215-573-7407 lpzhang@pennmedicine.upenn.edu 502-503 Stellar-Chance Bldg.
         
Jennifer Rosado Research Specialist 215-573-7410 jmrosado@pennmedicine.upenn.edu 502-503 Stellar-Chance Bldg.
Deborah Evans Financial Coordinator 215-573-7407 dlmckinn@pennmedicine.upenn.edu 502-503 Stellar-Chance Bldg.

Location and hours of operation

Hours Location

Monday - Friday     

9 AM - 5 PM 

502-503 Stellar-Chance Bldg 

422 Curie Blvd.

Philadelphia, PA 19104

Links and Resources

  1. https://genetics.med.upenn.edu/cores/genomic-analysis-core/
  2. https://www.med.upenn.edu/cores/
  3. https://www.pennmedicine.org/cancer/cancer-research/for-researchers/shared-resources

Contacts

Name Role Phone Email Location
Tapan Ganguly
Director
 
215-573-7238
 
gangulyt@pennmedicine.upenn.edu
 
B16 Anat-Chem Bldg.
 
Erik Toorens
Lab Manager
 
215-573-4832
 
toorens@pennmedicine.upenn.edu
 
Stellar Chance 502